MOLECULAR CLONING AND NUCLEOTIDE SEQUENCE OF 5′FRAGMENT OF ORF?2a GENE OF POTATO LEAFROLL VIRUS CHINESE ISOLATE
Bibliographic record
Abstract
According to the genomic sequence of four foreign PLRV isolates, a pair of specific primer was designed and synthesized.The c?DNA of 5′fragment of the ORF?2a gene of PLRV-Ch was synthesized by reverse transcription and followed by Polymerase Chain Reaction amplication.The synthesized 5′c?DNA fragment of the PLRV-Ch ORF?2a gene was inserted into pUC19 and cloned in E. Coli JM?109 and the sequence was determined.The homology of nucleotide sequence between PLRV-Ch and PLRV-S(Scotland,UK),PLRV-N(Netherlands),PLRV-A(Australia) and PLRV-C(Canada),were 98.20%,98.52%,96.40% and 97.55%.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".