EFFECTS OF OXYGEN FREE RADICALS ON K~+ CHANNELS IN WB-F344 CELLS
Bibliographic record
Abstract
Oxidant-induced damage has been implicated in the pathogenesis of several forms of cellular injury. In the present study cell-attached clamp technique was employed to determine if oxidant stress leads to activation of plasma membrane K+-channels in rat liver oval cell strain WB-F344. We found that, 1) Under experimental conditions (bath: Krebs, Pipette: High K+), if WB cells were stimulated directly by O2.- generated by interaction of Xanthine with Xanthine Oxidose (X:100μmol/L, XO:0.1mu/ml), channel activity was found. However, if 20mmol/L CaCl2 were exposed to cell bath, a small amplitude channel activity was recorded with the channel conductance 9.48±0.93ps(n=4). If CaCl2 and O2.- were exposed to cell bath, large amplitude channel activity was shown. In positive testing potential voltage, the activated channel conductance was 15.74±5.46ps(n=8); In negative testing potential voltage, the channel conductance was 48.32±8.67ps(n=6). 2) In ion replacement experiments these channels appeared as potassium channels. Potassium channels blocker 4-AP (2mmol/L) inhibited the currents and SNP (10nmol/L) increased the currents. These result suggests that activation of K+-channels seems to be important for the signal transduction of cellular responses to oxidant stress.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.003 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".