Effect of High Intensity Pulsed Electric Field on the Membrane Permeability and DNA of E coli
Why this work is in the frame
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Bibliographic record
Abstract
This paper studies the effects of different electric field strength and treatment time on the inactivation of Ecoli suspended in sodium chloride(electric conductivity δ=340μS/cm,30°C) by a continuous high intensity pulsed electric field(PEF) treatment system.The plate counting method is used to determine the viability of the cells.The results show that the survival of Ecoli decreases with increasing the electric field strength and treatment time,and E.coli is inactivated by 30 kV/cm,266 ms treatment.The subsequent leakage of protein and nucleic acid of Ecoli due to the PEF processing is investigated by using the ultraviolet spectrophotometric method.And the relative amounts of the leaked protein and nucleic acid from Ecoli increase after the PEF treatment,and increase with increasing the electric field strength and treatment time,showing that PEF treatment increases the membrane permeabilization of Ecoli.ERIC(Enterbacteria Repetitive Intergenic Consensus)-PCR method is used to determine the inactivation of PEF on Ecoli.ERIC-PCR fingerprinting demonstrated that DNA of the treated Ecoli is injured and DNA degradation appeares when the electric field strength is up to 20kV/cm for 539ms or more severe PEF treatment(30kV/cm for 266ms;35kV/cm for 266ms;50kV/cm for177ms),and macromolecule DNA is degraded into small molecule fragments.The results indicate the increased membrane permeability and DAN degradation may resulted in the lethal of Ecoli.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it