Clone the conserved sequence of CDPK from Glycine soja and analysis the sequence
Bibliographic record
Abstract
CDPK(Ca2+-dependent,calmodulin independent protein kinase) is the crucial protein kinase in plant calcium signal transduction,and play important role in osmotic stresses.Here based on the strategy with homologous sequences clone new gene.According to the published DNA sequences,alignment them,designed two pairs nest-primers in the higher homologous regions of CDPKs,combinated touch-down PCR and nest PCR to clone the conserved sequence of CDPK from Glycine soja.Eventually have gotten wild soybean CDPK conserva-tive sequence,through analysing,it included the conserved catalytic domain that is typical of serine/threonine protein kinases and a Ca2+-binding domain that contains three typical EF hand structures those different from other protein kinases.What′s more,the DNA sequence exhibited significant similarity to VrCDPK(U08140),reaches 94% similarity.Result has proved,also have CDPK gene in wild bean,and have established foundation to get the all length genes through the methods such as RACE in wild soybean.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.004 | 0.002 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".