Ionic and Osmotic Mechanisms Of Insect Chill-Coma And Chilling Injury
Bibliographic record
Abstract
A mechanistic understanding of how temperature limits insect performance is needed to accurately model insect distribution and abundance. Upon crossing the temperature of their critical thermal minimum (CTmin), insects enter a state of paralysis (chill-coma). Chill-susceptible insects accumulate injuries (termed chilling injury) during prolonged exposure to low temperatures. My objective was to determine the mechanisms by which both chill-coma and chilling injury manifest in chill-susceptible insects. In aquatic animals, critical thermal limits are associated with a temperature-induced failure of oxygen supply relative to demand (oxygen- and capacity- limitation of thermotolerance; OCLT), which leads to reliance on anaerobic metabolism at thermal extremes. However, using open-flow respirometry and biochemical techniques, I found that fall field crickets (Gryllus pennsylvanicus) in chill-coma continued to exchange gases through the tracheal system and did not accumulate anaerobic byproducts, which suggests OCLT does not set the CTmin of insects. To characterize the patterns of ion balance disruption at low temperatures, I estimated water and ion content of the hemolymph and tissues of G. pennsylvanicus in chill-coma using gravimetric methods and atomic absorption spectrometry. Exposure to low temperatures caused a movement of Na+ and water from the hemolymph to the gut in G. pennsylvanicus, which increased hemolymph [K+] and depolarized muscle resting potential. When removed from the cold, crickets rebalanced ions and water, and the restoration of hemolymph [K+] (and muscle equilibrium potential) was coincident with the recovery of neuromuscular function. Although crickets recover the ability to move rapidly after removal from the cold, complete recovery of ion and water homeostasis requires additional time and metabolic investment. There is both inter- and intraspecific variation in cold tolerance in flies of the genus Drosophila. Using ion-selective microelectrodes, I found that cold-tolerant Drosophila species and cold- acclimated D. melanogaster maintain low concentrations of [Na+] and [K+] in their hemolymph. Drosophila cold tolerance was also associated with low Na+/K+-ATPase activity on a whole-organism level. Together, these studies allow me to construct a conceptual model of how the direct effects of temperature on ion homeostasis may drive chill-coma, chill-coma recovery and chilling injury in insects.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".