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Record W2370330591

Cloning and sequencing of NS2 gene mRNA of Porcine parvovirus

2010· article· en· W2370330591 on OpenAlexaboutno aff
Zhenmei Zhang

Bibliographic record

VenueHeilongjiang xumu shouyi · 2010
Typearticle
Languageen
FieldAgricultural and Biological Sciences
TopicAnimal Virus Infections Studies
Canadian institutionsnot available
Fundersnot available
KeywordsBiologyGeneMolecular biologyMessenger RNAPorcine parvovirusComplementary DNAGene expressionParvovirusCloning (programming)Homology (biology)GeneticsVirologyVirus
DOInot available

Abstract

fetched live from OpenAlex

To provide a new ideas for the study of the mechanism of porcine parvovirus replication,the experiment used PPV-BQ strain of porcine parvovirus vaccinated subculture ST cells,extracted the total RNA when the virus replicated in the process of ST cells,and then reversed transcription into cDNA.mRNA fragments of NS2 gene about 486 bp and Pre-mRNA fragment of the NS2 gene about 1 959 bp were amplified by RT-PCR.Each of the gene was cloned into the vector pMD18-T and sequenced.The results showed that mRNA fragments in NS2 gene of PPV-BQ strain shared 97.4%~99.4% and 94.4%~98.8% homology with published in GenBank at nucleotide sequence and amino acid level,respectively.According to phylogenetic analysis,PPV-BQ strain has a close genetic relationship with SR-1 strain isolated in Si-Chuan,Kresse strain isolated in Canada and NADL-2 strain isolated in USA.Acquired NS2 gene mRNA during replication lay the foundation for the research of gene expression in NS2 protein and its function.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.006

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0000.001
Bibliometrics0.0010.001
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0020.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.024
GPT teacher head0.227
Teacher spread0.203 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2010
Admission routes1
Has abstractyes

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