Regeneration of Rice Mutants Harbouring Ac/Ds Elements Via Tissue Culture
Bibliographic record
Abstract
The rice seeds,which were confirmed to harbour both Ac and Ds elements,were used as experimental materials to perform tissue culture.Regenerated plants(R0) were obtained.After transfer to soil and growing,mature seeds(R1) were harvested from regenerated plants.Leaf sampling was carried out when the regenerated plants grew vigorously at tillering stage,and the genomic DNA extraction was conducted from these sampled leaves.The extracted genomic DNA was adopted as template in a PCR using specific primers to check the Ac/Ds insertion,Ac/Ds insertion pattern was screened by PCR from a population of 111 plants regenerated from seeds harbouring both Ac and Ds.The result showed that 94% carried both Ac and Ds elements,while 6% Ac alone.The Ac plants were devoid of Ds element,the cause of which remains unclear and needs further work.Thus,it is suggested that enlarging the mutant library via tissue culture should be feasible in rice.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".