Optimization of Cryopreservation Condition for Paris polyphylla var. chinensis
Bibliographic record
Abstract
Objective: To establish the critical techniques for cryopreservation of Paris polyphylla var. chinensis.Method: The concentration of dimethyl sulfoxide,pre-cultivation time,loading time,and dehydration time were optimized in an orthogonal design. Four different levels were tested for each of the factor. The results were evaluated by the relative survival rate of the cells. Result: The optimal cryopreservation condition for Paris polyphylla var. chinensis was pre-cultivation in 5% DMSO for 5 days,followed by 45 minutes loading with 60%vitrified solution. The sample was then dehydrated with 100% vitrified solution for 1 hour. Under such condition,the relative survival rate was 92. 10%. Conclusion: The method of vitrification to cryopreserve the germplasm of P. polyphylla var. chinensisis simple in handling with high in survival and can be applied in practice.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".