Identification and Control of the Endogenous Microbe in Anti-Antibiotic Screening of Transgenic Brassica napus Seeds
Bibliographic record
Abstract
Arabidopsis Auxin Response Factor 8(AtARF 8) gene can weaken the apical dominance and improve side sprouts growing when it is overexpressed.The flore-dip transformation method was used to transfer AtARF 8 gene into rape(Brassica napus L.) flowers and 8 143 candidate seeds were harvested from transformed plants.During screening for the transgenic plant,however,250 mg/L kanamycin was not able to effectively control contamination of endogenous microbe from seeds.Through cytological identification and PCR technique,endogenous microbe were determined to be agrobacterium LBA 4404.Different antibacterial substances such as gentamicin sulphate and ceftriaxone sodium were employed to eliminate contamination resulted from the endogenous bacteria.It was found that 50 mg/L ceftriaxone sodium could not only control the contamination but also had little effects on the growth and development of the plant.So 50 mg/L ceftriaxone sodium can be used as the appropriate antibiotic in anti-antibiotic screening of transgenic Brassica napus seeds.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".