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Record W2376868513

Establishiment of cDNA-AFLP Analysis System for Melon

2009· article· en· W2376868513 on OpenAlexvenueno aff
Qin Jianbing

Bibliographic record

VenueSeed · 2009
Typearticle
Languageen
FieldEnergy
TopicPower Systems and Renewable Energy
Canadian institutionsnot available
Fundersnot available
KeywordsMelonTrizolCucumisComplementary DNAAmplified fragment length polymorphismBiologyMolecular biologyChemistryGeneHorticultureBiochemistrycDNA library
DOInot available

Abstract

fetched live from OpenAlex

The key factors affecting cDNA-AFLP analysis system of cucumis melon was studied,and an optimized system was established which showed a distinct electrophoretogram of cDNA-AFLP.The result indicated that the total RNA from melon using Trizol reagent was good for this study and replacement was sit for synthesizing dsCDNA.The two pairs restriction enzyme combinations were good for melon both although EcoR Ⅰand MseⅠ was better.The dosage of dscDNA was 300 ng,the pre-amplification procducts using template of select-amplification were diluted to 30 times and else were perfect for melon.Otherwise,8% no-denaturalization polypropylene gel for electrophoresis and display with siller were simpler and more facility than denaturalization polypropylene gel that literatures shown.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.433
Threshold uncertainty score0.554

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.001
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.009
GPT teacher head0.226
Teacher spread0.218 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2009
Admission routes1
Has abstractyes

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