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Record W2377014352

Determination of the partial polymerase gene sequence of novel coronavirus isolated from lung tissue of SARS patients

2003· article· en· W2377014352 on OpenAlexaboutno aff
Qin Ede

Bibliographic record

VenueBulletin of The Academy of Military(Medical Sciences) · 2003
Typearticle
Languageen
FieldAgricultural and Biological Sciences
TopicAnimal Virus Infections Studies
Canadian institutionsnot available
Fundersnot available
KeywordsPolymerase chain reactionHomology (biology)BiologyGeneCoronavirusVirologyPolymeraseSequence analysisReverse transcription polymerase chain reactionMolecular biologyPhylogenetic treeDNA polymeraseGeneticsCoronavirus disease 2019 (COVID-19)Gene expressionMedicinePathology
DOInot available

Abstract

fetched live from OpenAlex

Objective:To amplify and determine the sequence of partial polymerase gene of novel coronavirus isolated from lung tissue of severe acute respiratory syndrome(SARS)patients in mainland China, and to confirm SARS pathogen.Methods:DNA fragments of the polymerase gene were obtained by reverse transcription_polymerase chain reaction (RT_PCR) and the sequence homology and phylogenetic were analysed by the Neighbour Joining method of Clustal X 1.8.Results and Conclusions: The DNA fragments with expected length could be amplified by RT_PCR from the lung tissue of SARS patients and that of infected mouse and the cell cultures. The sequence homology analyses revealed that the sequence had about 60% homology compared with other known coronaviruses, and had 99% homology with novel coronaviruses isolated in USA and Canada.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.001
Threshold uncertainty score0.003

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.056
GPT teacher head0.297
Teacher spread0.241 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations4
Published2003
Admission routes1
Has abstractyes

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Same venueBulletin of The Academy of Military(Medical Sciences)Same topicAnimal Virus Infections StudiesFrench-language works237,207