Functional analysis in the upstream cis-regulatory elements and introns of bovine skeletal α-actin gene
Bibliographic record
Abstract
Bovine skeletal muscleα-actin mutation promoters were constructed by PCR site-specific mutagenesis to determine the functions of Sp1/KLFs,ZF5 F,Pax3binding sites located at-361--462bp;the reformed promoters containing introns were constructed by gene recombination technology.The dual luciferase expression vector were transfected to skeletal muscle satellite cells and bovine fetal fibroblast cells for activity detection.The results showed that,Sp1/KLFs could decrease promoter activity,ZF5 Fand Pax3could increase promoter activity.Introns located at the upstream of promoter increased the promoter activity,located at the downstream of promoter decreased the promoter activity.Cis-regulatory elements and introns had muscle-specificity.Analysis by PCR site-specific mutagenesis showed that cis-acting element Pax3 possessed as transcriptional repressor,Sp1/KLFs and ZF5 Fpossessed as transcriptional activators.It is found that the infulence of intron on bovine skeletalα-actin promoter activity is related to its location,which has laid a foundation for construction of bovine skeletalα-actin efficient promoter.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".