Bibliographic record
Abstract
The mature peptide coding sequence of xylanase gene xynB(746 bp)was amplified from the Aspergillus niger CICC2462,for high expression of glucoamylase gene locus in Aspergillus niger CICC2462, Splicing xynB gene fragment, glucoamylase gene 5 'homology arm and 3' homologous arm for homologous recombination expression cassette GBG by overlap extension PCR,The whole gene was inserted into expressional vector pSZH-CYM form the Aspergillus niger expressional vector pSZH-xynB, Using freeze thawing method pSZH-xynB expressional vector was transformed into Agrobaoterium tumefaciens AGLI, Thus transformed Aspergillus niger CICC2462 mycelium by agrobacterrium-mediated transformation. Detection 47 positive transgenic strains by hygromycin selection and PCR,Screening 9 homologous recombinant strains which occurrence gene replacement at the glucoamylase gene locus from 12 strains. Then through successive generations, screening and PCR to obtain eliminate hygromycin gene homozygous homologous recombination strains, thereby obtain the production requirements of the foodgrade engineering strain. Engineering strain showed that glucoamylase fermentation condition of the engineering strain culture supernatant xylanase activity up to 4495.8975 U · mL-1by fermentation culture, detection of enzyme activity and SDS-PAGE analysis. The results of this study provide a new way for production low-xylosidase activity of endo-xylanase, and also provide a valuable reference for the safe and efficient production other enzymes and recombinant protein expression system to take advantage of Aspergillus niger.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".