Inrestigation on the purification methods of β-mannase from Bacillus subtilis WD-23 and studies on enzymatic properties
Bibliographic record
Abstract
The objective of this study was to purify β-mannase from Bacillus subtilis WD-23 and examine its enzymology properties. This study used the combining methods of ammonium sulfate,semi-permeable membrane dialysis,polyethylene glycol concentration,DEAE-Sepharose FF ion exchange chromatography,Sephadex G-75 gel filtration and SDSPAGE gel electrophoresis to purify β-mannase. The result showed that the purified ratio of β-mannase of B. subtilis WD-23 was 14. 1 by using the above methods,and the molecular mass was about 40 ku,the optimal pH and the stability range of pH were 5. 6 and 5. 0- 7. 0 respectively,and optimal temperature and the stability range of temperature were 55 ℃ and 40- 70 ℃ separately,Ca2 +was the most obvious effect on the activation of the enzyme,on the contrary,the inhibitory effect of Li+was the most obvious.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.002 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".