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Record W2377479133

Cloning and Functional Analysis of Bovine Desmin Gene Promoter

2014· article· en· W2377479133 on OpenAlexaff
Du We

Bibliographic record

VenueXumu shouyi xuebao · 2014
Typearticle
Languageen
FieldMaterials Science
TopicSilk-based biomaterials and applications
Canadian institutionsScience North
Fundersnot available
KeywordsDesminBiologyMolecular biologyGeneTransfectionReporter genePromoterGene expressionGeneticsVimentin
DOInot available

Abstract

fetched live from OpenAlex

This study aimed to clone bovine desmin gene promoter fragments of different lengths,and analyze its function.The bovine desmin gene promoter deletion sequences of different lengths were amplified by PCR,pGL3-desminpro dual luciferase expression vector transfected to skeletal muscle satellite cells and bovine fetal fibroblasts for activity detection was constructed.Meanwhile bovine MyoG and α-actin genes promoter expression vectors were transfected in order to compare the desmin,MyoG and α-actin genes promoter expression activity.The results showed that bovine desmin gene in the range of 132-2 106 bp promoter could start the expression of dual luciferase reporter gene at different degrees in bovine skeletal muscle satellite cells,and had muscle-specific.300 bp desmin gene promoter had the highest activity and higher than that of MyoG and α-actin genes promoter activity in bovine.Thus the 300 bp desmin gene promoter activity was higher and was adaptive to the muscle-specific promoter,which has laid an important foundation for the production of transgenic bovine.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesInsufficient payload (model declined to judge)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.062
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.018
GPT teacher head0.249
Teacher spread0.231 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2014
Admission routes1
Has abstractyes

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