Bibliographic record
Abstract
This study aimed to clone bovine desmin gene promoter fragments of different lengths,and analyze its function.The bovine desmin gene promoter deletion sequences of different lengths were amplified by PCR,pGL3-desminpro dual luciferase expression vector transfected to skeletal muscle satellite cells and bovine fetal fibroblasts for activity detection was constructed.Meanwhile bovine MyoG and α-actin genes promoter expression vectors were transfected in order to compare the desmin,MyoG and α-actin genes promoter expression activity.The results showed that bovine desmin gene in the range of 132-2 106 bp promoter could start the expression of dual luciferase reporter gene at different degrees in bovine skeletal muscle satellite cells,and had muscle-specific.300 bp desmin gene promoter had the highest activity and higher than that of MyoG and α-actin genes promoter activity in bovine.Thus the 300 bp desmin gene promoter activity was higher and was adaptive to the muscle-specific promoter,which has laid an important foundation for the production of transgenic bovine.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".