Expression of VP2 Protein of Infectious Bursal Disease Virus HB Strain and Analysis of Immunogenicity
Bibliographic record
Abstract
The aim of this study is to identify the immunogenicity of the VP2 protein of infectious bursal disease virus(IBDV) named as HB strain.The cloned genomic DNA of VP2 gene was sub-cloned into prokaryotic expression vector PET-32a-(+),then the recombinant protein was induced and expressed,a 55 ku protein was analyzed by SDS-PAGE and Western blot.Six week old Balb/c mice were immunized with purified recombinant protein,and all the Balb/c mice produced antibodies,the antibody titer was more than 1∶6 400.The results showed that the recombinant protein expressed in E.coli still maintained good antigenicity of IBDV antigens.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".