Constructing a pre--erythrocytic multi--epitope chimeric antigen of Plasmodium falciparum and its expression in Pichia pastoris
Bibliographic record
Abstract
Objective:To construct a pre erythrocytic mult i epitope antigen of Plasmodium falciparum (designated as PfCP TCL ) and express it in Pichia pastoris in secreting form. Methods: Some importa nt T and B epitopes from pre erythrocytic antigen candidates such as CSP, TRAP a nd LSA 1 were selected and joined in tandem to generate the chimeric gene. The gene was expressed in Pichia pastoris and its product was purified by chroma togr aphy. Results: PfCP TCL gene was synthesized and expressed at h igh level of more than 1 g/L. The product was purified by ion exchange and gel filter chromatography with a purity of 95%. Conclusi on : The synthetic PfCP TCL gene is expressed in Pichia pastoris and a method of easy purification is developed. The recombinant PfCP TCL protein provides a base for investigating its immune function and potential as a component of combined malaria vaccine.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".