Identification of a novel G-protein Gαq gene in shrimp(Litopenaeus vannamei)
Bibliographic record
Abstract
In this study,we aimed to identify new functional genes encoding G protein alpha subunits from Litopenaeus vannamei and elucidate the critical roles that Gα subunits assume in a signal transduction process of shrimp.Degenerate PCR and RACE techniques were employed to achieve the full length of Gα subunit in L.vannamei.Co-immunoprecipitation analysis and measurement of second messengers of Ca2+ and IP3 in HEK 293 cells overexpressed with this new Gα subunit were used to identify the basic functions.Western blotting analysis was employed to detect the distribution of the Gα subunit.In the results,the predicted encoding protein of the novel cDNA sequence shared high similarity to Gαq from other organisms,and was thus termed pvGαq.pvGαq was highly conserved in both primary structures and basic functions.The expression pattern revealed that pvGαq was widely expressed in many shrimp tissues,especially enriched in brain and eyes,it also obviously existed in gills,maxillas and antennae.These results indicated that pvGαq may play critical roles in sensory responses and nerve system of shrimp L.vannamei,which provided a basis for the research of physiological regulation of shrimp.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".