Saturated fatty acids trigger apoptosis and inhibit cell cycle progression in insulinoma cells
Bibliographic record
Abstract
Objective To investigate the effect of saturated fatty acid palmitate acids(PA) action on viability,proliferation and apoptosis of murine insulinoma MIN6 cells,and the possible intracellular pathways activated by PA.Methods Cell viability was evaluated with MTT reduction conversion assay.The Annexin-Ⅴ/FITC cell death detection kit was used to monitor PA-induced apoptosis.(IAP) family(XIAP and cIAP-2),cyclin D1 and CDK4 were further detected using Western blot.Results ① PA of different concentration significantly inhibited the proliferation of MIN6 cells(P0.01).② PA increased MIN6 cell apoptosis in time-and concentration-dependent manners(P0.01).③ The G0/G1 cell cycle arrest was also induced by PA(P0.01),whereas MIN6 cells in S and G2/M phase were decreased(P0.01).④ Western blot revealed that PA reduced the level of AKT phosphorylation(P0.01),decreased the expression of XIAP(P0.01) and cIAP-2(P0.01) compared to BSA-treated control cells.PA also reduced protein expression of cell cycle-related molecules,CDK4(P0.01),and cyclin D1(P0.01) compared to BSA-treated control cells.Conclusion Our results thus suggest that PA directly affects pancreatic β-cell cell proliferation,possibly by inhibiting Akt kinase and reducing the level of cyclin D1/CDK4 in the cells,which results in arresting in progression through the G1 phase to the S phase of the cell cycle.PA triggers pancreatic β-cell cell apoptosis,possibly by reducing the levels of XIAP and cIAP-2 in the cells.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".