Detection of Vitellin Concentration Changes during Embryonic and Laval Development of Redclaw Crayfish,Cherax quadricarinatus,by ELISA
Bibliographic record
Abstract
An indirect competitive enzyme linked immunosorbent assay(ELISA) for detecting vitellin in embryonic and laval Redclaw Crayfish,Cherax quadricarinatus was developed.This method was sensitive to the vitellin,with the effective range of 31.25-250 ng/ml.The results indicated that,during embryonic development,proteins with a molecular weight larger than that of vitellin were degraded,and both large and small molecular weight protein subunits were consumed first.The concentration of vitellin decreased during development.Its concentration was a little increased at cleavage stage and blastula stage(3.19%),reached the highest level in nauplius stage(4.67%),and then decreased remarkably when the laval could live independently,at which time the concentration of the vitellin was only 1/4 of that in fertilized eggs.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".