Improvement of Separation and Purification of Polyphenol Oxidase from Tobacco
Bibliographic record
Abstract
Objective To find out the optimal method purifying polyphenol oxidase(PPO) from tobacco. Method PPO was purified through the different saturated concentration salt-out DEAE-Sepharose Fastflow and Sephadex G-150. The optimal method was obtained through mensurating and comparing the activity of PPO. Results The optimum ammonium sulfate concentration of salt-out is 40% and it enhanced the time of purification to 8.4 fold. The optimal method purifying PPO through DEAE-Sepharose Fastflow and Sephadex G-150 was obtained. The relative molecular weight of purified protein is 42 000 and Km is 1.2 mmol/L therefore it was identified as the polyphenol oxidase Ⅱ. Conclusion For the manipulative steps this experiment omitted the following steps organic solvent fractional precipitation cation-exchange chromatography and so on and it also shorten the process greatly.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".