The Screening and Identification of Transgenic Goat Fibroblast Cells with Sweet Protein Brazzein Gene
Bibliographic record
Abstract
[Objective]In order to obtain transgenic goat fibroblast cells with sweet protein Brazzein gene. [Method]Goat fibroblast cells were transfected with mammary gland specific expression vector STP-pBC1 constructed in the previous work by cationic liposome method and cultured G418-resistant cell. After PCR identified,transgenic cell line with Tem was established. The G418 positive cells were detected. [Result]Transgenic cell could be obtained by the optimum concentration of G418. Morphology of the transgenic cell and transgenic cells after freezing-thawing was similar with normal mammary epithelial cells,the cell growth curve wasSshape,PCR results showed that the vector constructed was integrated into genome. [Conclusion]The transgenic cells with temporin-GFP were filtrated,and lay the foundation for the transgenic goat with expression temporin in mammary.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".