Bibliographic record
Abstract
Castor seed size varies greatly due to breed,has yet to see molecular biology research on castor bean size traits associated with the development. In this experiment,32 castor seeds of different sizes as research material,with U25( 55) uniform design for seed size traits RAPD-PCR reaction system and conditions were optimized. The optimal reaction system: 25 μL system,10 × Buffer 2. 7 μL,d NTP 2. 4 μL,Mg2 +2. 4 μL,DNA 1. 7 μL,primers 1. 9μL,Taq polymerase 2. 0 μL,dd H2 O 11. 9 μL. The best reaction conditions were: 94 ℃ 150 s; 94 ℃ 45 s,39 ℃65 s,72 ℃ 90 s,35 cycles; 72 ℃ 10 min; 4 ℃ preservation. With SB-002 primer( nucleotide sequence( 5'-3') :TGCCGAGCTG),using the best reaction system and conditions,screening a different band in the largest grain castor seeds,sequencing results were compared in the NCBI found with high yield rice genome DNA,mRNA or c DNA homology of 100%. Therefore,It can be concluded that the different band relevant to castor seed yield,laid the foundation for the relevant molecular mechanisms of castor seed size traits studied.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.002 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".