Bibliographic record
Abstract
Objective:The purpose was to express porcine phospholipase gene(PLA2,PLA2M) in Aspergillus niger.Method:The study constructed porcine phospholipase A2 recombinant expression vector pSZHG-PLA2,pSZHGS-PLA2M,which contained its own signal peptide or glucoamylase signal peptide.pSZHG-PLA2 and pSZHGS-PLA2M were transformed into Aspergillus niger by Agrobacterrium-tumefacien mediated method.Result:After screened,respectively,both pSZHG-PLA2 and pSZHGS-PLA2M obtained a homologous recombination transformant.To screen out homologous recombination strains for fermentation,respectively,detected the intracellular enzyme activity and extracellular enzyme activity.The result showed that the intracellular and extracellular enzyme activity of homologous recombination strain,which guided by its signal peptide,were 21.68U/mL and 2.12 U/mL.The intracellular and extracellular enzyme activity of homologous recombination strain,which guided by glucoamylase signal peptide,were 15.07U/mL and 3.10 U/mL.Conclusion:The results showed that the study used of its signal peptide and glucoamylase signal peptide had failed to obtain effective secretion of the recombination porcine phospholipase A2 gene in Aspergillus niger.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".