CONSTRUCTION OF PLANT EXPRESSION VECTORS CONTAINING Aedes aegypti TMOF ANALOGUE GENES,AND THEIR TRANSFORMATION TO TOBACCO
Bibliographic record
Abstract
Trypsin-modulating oostatic factor(TMOF) can inhibit trypsin activity in insect midgut dramatically.To analyze inhibitory ability of Aedes aegypti TMOF analogues genes to insect,we reconstructed plant expression vectors containing Aedes aegypti TMOF analogue genes,and then transformed those into tobacco.The synthetic Aea-TMOF analogues were cloned into modified PCAMBIA1301 vector that contains constitutive promoter CaMV35S.The recombinant plasmids of PCAMBIA1301 + Aea-TMOF were transferred into Agrobacterium tunefacien LBA4404 by freeze-thaw method;and then were used to infect tobacco leaf discs.Using PCR and GUS methods,we proved that the Aea-TMOF analogue genes were integrated into tobacco genome.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".