Sensitivity of BmHc Cell Line From Bombyx mori to Lipopolysaccharide and 20-hydroxyecdysone
Bibliographic record
Abstract
To study signal transduction path way in intracellular cells of insect and express foreign gene in cultured hemocytes,a BmHc hemocytes cell line from B.mori was established in TC-199 medium.The BmHc cell line consists of all types of hemocytes including prohemocytes,spherulocytes,granulocytes and plasmatocytes,etc.Doubling time of the cell line is about 30 h.LPS elicited hemocytes to synthesize antibacterial proteins and peptides and prompted cells to uptake more Ca2+ to take part in active cell proliferation.Flu-3 AM study with TCS-SP2 laser scanning confocal microscope showed that Ca2+ distributed on the membrane of prohemocytes and granulocytes,but some of them were on the signal path way to the prohemocytes nuclei in 30 min after LPS induction;while under the same condition,the membrane of the cells was only slightly stained with Flu-3 AM after treatment with 20E.LPS elicited and opened Ca2+ channel and caused the change of.A relatively stable change of in the cells when 20E was added to the culture medium that indicated 20E is not an effective enhancer for sensitive Ca2+ channel on the cell membrane.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".