Effects of Supernatant of CLEC2B Gene Overexpression in Jurkat Cells on the B16 Melanoma cell
Bibliographic record
Abstract
Objective The melanoma cells were treated with culture supernatant of CLEC2B gene overexpression in Jurkat cells,to observe the impact of melanoma proliferation,the activity of tyrosinase and melanin synthesis,for searching CLEC2B gene participated in the vitiligo.Methods In this study,Jurkat cells were transfected with recombinant plasmid pGCMV/EGFP/Neo-CLEC2B by DMRIE-C Reagent.RT-PCR identified CLEC2B gene overexpression.After 48 h transfection,the melanoma cells were treated with their culture supernatant for 48 h,then the proliferation of melanoma cells were detected by MTT,the activity of tyrosinase was detected by Dopa oxidation,melanin synthesis was detected by Sodium hydroxide lysis.Results The proliferation rate of melanoma cells in CLEC2B gene transfection group was significantly lower than the empty vector group and control group(P 0.05),the activity of tyrosinase of melanoma cells with among CLEC2B gene transfection group,empty vector group and control group had no significant difference(P 0.05),the melanin content of melanoma cells with in CLEC2B gene transfection group was significantly less than the empty vector group and control group(P 0.05),the empty vector group had no significant difference with the control group(P 0.05).Conclusion The Jurkat cells culture supernatant with CLEC2B gene overexpression could inhibit the proliferation and melanin synthesis of melanocytes in some extent,it has no apparent effect on the activity of tyrosinase of melanocytes.CLEC2B could influence the proliferation and melanin synthesis of melanocytes by the way of affecting lymphocytes’function.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".