Effects of transplantation of tissue inhibitor-3 of matrix metalloproteinase gene-transfected vascular smooth muscle cells on heart structure and function after myocardial infarction
Bibliographic record
Abstract
AIM: To investigate the effects of transplantation of tissue inhibitor-3 of matrix metalloproteinase(TIMP-3) gene-transfected vascular smooth muscle cells(VSMCs) on heart structure and function after myocardial infarction(MI) in rats.METHODS: VSMCs were obtained from rat thoracic aorta by tissue-piece inoculation.TIMP-3 gene was transfected into VSMCs by lipidosome-mediated method.One hundred and twenty-one female Wistar rats,aged 12 weeks and weighing between 200 and 250 g,were prepared.Acute MI models were established in 93 rats by ligating the descending left coronary artery;84 survived and were divided randomly into three groups(n=28).Three days after MI,rat chests were opened again and 0.3 ml Dulbecco's modified Eagle's medium(DMEM) containing 3×106 TIMP-3 gene-transfected VSMCs(group A),3×106 VSMCs(group B) or 0.3 ml DMEM(group C) was immediately injected into the infarcted myocardium.The control group(group D) consisted of 28 rats without any treatment.Heart function was detected by ultrasonic echocardiogram.Heart samples were harvested and heart left ventricular volume and volume index were measured.Heart structure was also observed by tissue morphologic examination.RESULTS: VSMCs were cultivated and had a high purity(98%).TIMP-3 gene was transfected into VSMCs successfully.Four weeks after gene transfer,heart function in group A was improved over group B and group C(P0.05),group B was improved over group C(P0.05),but they were all poorer than group D(P0.05.Left ventricular volume was(94.4±158.3) mm3,(1 126.5±284.7) mm3,(1 372.8±181.9) mm3 and(420.2±39.6) mm3 in the four groups,and volume index was(2.957±0.362) mm3/g,(3.604±0.710) mm3/g,(4.538±0.259) mm3/g and(1.009±0.134) mm3/g.The results of histological observation showed MI in group C.Clustering muscle cells were seen in infarction field in group B and heart ventricle wall was thicker than in group C.Infarction field in group A was smaller than in groups B and C.Heart ventricle wall in group A was thicker than in groups B and C.CONCLUSION: Implanted TIMP-3 gene transfected VSMCs in infarcted myocardium at 3 days after acute MI noticeably improves heart structure and function in rats.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".