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Record W2386410674

Expression of sIL-16 in E.coli and Its Activity Analysis

2002· article· en· W2386410674 on OpenAlexaff
Zhi Ye

Bibliographic record

VenueLife Science Research · 2002
Typearticle
Languageen
FieldImmunology and Microbiology
TopicGalectins and Cancer Biology
Canadian institutionsCAE (Canada)
Fundersnot available
KeywordsJurkat cellsRecombinant DNAlac operonMolecular biologyComplementary DNAAffinity chromatographyEscherichia coliExpression vectorProtein expressionPlasmidBiologyChemistryGeneBiochemistryT cellEnzymeGenetics
DOInot available

Abstract

fetched live from OpenAlex

The sIL16 cDNA was amplified by PCR,and then the fragment was inserted into an expression vector pET28a(+) under T7 promoter control and constructed recombinant plasmid pETsIL16.pETsIL16 was transformed to E.coli BL21(DE3) and the protein was expressed after IPTG induction at low temperature.It was purified by Ni2+ affinity chromatography column,and incubating with Jurkat cells to access its function to IL2R expression on the surface of the cells. An obvious expression band about 18kD can be detected in the recombinant protein by SDSPAGE.The purity efficiency was up to 90 percent.And the recombinant product upregulated the IL2R expression on surface of the Jurkat cells about 18.54 percent.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.008

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0010.001
Bibliometrics0.0000.001
Science and technology studies0.0000.000
Scholarly communication0.0010.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0020.003

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.099
GPT teacher head0.390
Teacher spread0.291 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2002
Admission routes1
Has abstractyes

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