Changes in Antioxidase Activity and Osmotic Adjusting Substance of Tamarix chinensis Seedlings under NaCl Stress
Bibliographic record
Abstract
To obtain tissue-cultured plant,We used Tamarix chinensis seeds as materials and established their sterile solated culture system by tissue culture method.A stress condition was formed through adding different concentrations of NaCl(0,50,80,100,120,150 and 200mmol/L)to the medium(MS+1.0mg/L6-BA+0.1mg/L NAA).To explore the physiological mechanism of T.chinensis tissue-cultured plant response to NaCl-stress,We investigated the changes of activities of T.chinensis tissue-cultured plant antioxidases,contents of osmotica,plasma membrane permeability and production rate of super oxygen anion(O-·2)under different concentrations of NaCl-stress.The results indicated that:(1)The activities of superoxide dismutase(SOD)and peroxidase(POD)of T.chinensis tissue-cultured plant were rising and thengoing down as concentrations of NaCl-stress were increasing,and both at 100mmol/L NaCl-stress reached a maximum,whereas,the activity of ascorbic acid peroxidase(APX)was gradually declining.(2)The contents of osmotica such as free proline(Pro),soluble protein(Pr)and soluble sugar of tissue-cultured plant were gradually rising and significantly higher than that of the concurrent control as concentrations of NaClstress were increasing,and at 200mmol/L concentration notably increased 53.77%,47.38% and 67.65%,respectively.(3)The production rates of hydrogen peroxide(H2O2)and super oxygen anion(O-·2),content of malonic dialdehyde(MDA)and plasma membrane permeability of tissue-cultured plant were all gradually rising and significantly higher than that of the concurrent control as concentrations of NaCl-stress were increasing,and at 200mmol/L concentration notably increased 36.42%,58.71%,82.60%and 42.54%,respectively.The study confirmed that antioxidases system of T.chinensis tissue-cultured plant showed favorable active oxygen scavenging activity under a low concentration of NaCl-stress(≤100 mmol/L)and the osmotica played positively regulated functions under different concentrations of NaCl-stress.Accordingly,T.chinensis showed a strong salt tolerance.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".