The Screening and Identification of Transgenic Goat Mammary Epithelial Cells with Temporin Gene
Bibliographic record
Abstract
In order to obtain Rana temporaria chensinensis skin antimicrobial peptide genes expressed in goat mammary epithelial cells,goat mammary epithelial cells were transfected with mammary gland specific expression vector Tem-GFP-pBC1 constructed in the previous work by cationic liposome method and cultured with G418-resistant cell.After PCR was identified,the transgenic cell line with Tem was established.The G418positive cells were detected.The results were as follows.G418-resistant cells could express GFP and Tem.Morphology of the transgenic cells after freezing-thawing was similar to that of normal mammary epithelial cells,the cell growth curve was inSshape,PCR results showed that the vector constructed was integrated into genome.Compared to the control,the transgenic cell could express Tem-GFP.The transgenic cells with temporinGFP were filtrated,and lay the foundation for the transgenic goat with expression temporin in mammary.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".