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Record W2387236504

Studies on Optimization of ISSR-PCR Reaction System for Bromus ciliatus L.

2009· article· en· W2387236504 on OpenAlexvenueno aff
Dai Jun

Bibliographic record

VenueSeed · 2009
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicBiopolymer Synthesis and Applications
Canadian institutionsnot available
Fundersnot available
KeywordsPrimer (cosmetics)DNAChemistryReaction conditionsgenomic DNABromusBiologyBotanyBiochemistryOrganic chemistry
DOInot available

Abstract

fetched live from OpenAlex

In this paper,the main indices of ISSR were studied taking the genomic DNA extracted from Bromus ciliatus L.as template.The optimum reaction system and reaction process of ISSR were found.The results showed that the reaction system was 20 μl.It included 2.0 mmol/L Mg2+,2.0 U Tap enzyme,0.3 mmol/L dNTP,0.5 μmol/L primer,30 ng DNA template.The reaction program was devised for 5 minutes at 94 ℃ in the first circle,45 seconds at 94 ℃ for 45 cycles,54 ℃ for 60 seconds,72 ℃ for 90 seconds,and 72 ℃ for 7 minutes in the final cycle.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.033
Threshold uncertainty score0.222

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.017
GPT teacher head0.274
Teacher spread0.256 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2009
Admission routes1
Has abstractyes

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