Expression of Hepatitis B Virus Surface Antigen in Nicotiana benthamiana Using Potato Virus X Vector
Bibliographic record
Abstract
Objective: Using plant viral expression vector potato virus X(PVX),the hepatitis B virus surface antigen(HBsAg) was expressed in Nicotiana benthamiana.It would become potential prospective in producing plant vaccine.Methods: The HBsAg gene was cloned into PVX viral expression vector,and electrotransformed into Agrobacterium tumefaciens,then inoculated leaves,stems and roots.Results Conclusion: The expression level of the recombinant HBsAg protein was determined with ELISA,and its size wasconfirmed by SDS-PAGE and Western blot.The age of leaves significantly affected the expression of HBsAg protein,as that the younger leaves accumulating 267.81 ng/mL which is 2～60 times the concentration in older leaves.The recombinant protein accumulation within a given leaf declined slightly over time but was not significantly different between 11 and 21 days post-inoculation,and the highest expression level was 796.81 ng/mg total soluble protein.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".