Cloning of Coding Sequence of Glutamin Synthetase from Polygonum sibiricum Laxm. and Its Expression under Alkali Salinity Stress
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Bibliographic record
Abstract
The full-length of Glutamin synthetase( termed PsGS) gene was cloned using RACE( rapid amplification of cDNA end) technology based on GS partial sequence obtained from a random clone in SSH library of Polygonum sibiricum Laxm.. The acquired 1 273 bp sequence includes a 5' untranslated region of 178 bp,a 3' untranslated region of 24 bp with poly( A),and an open reading frame( ORF) encoding 356 amino acids. Based on the comparison with amino acid sequences of other plant glutamin synthetase and the phylogenetic analysis of protein evolution,this gene was divided into glutamin synthetase family. The expression analyses by RT-PCR showed that PsGS expressed in leaves,stems and rhizomes of P. sibiricum Laxm.. Under the induction of 3% NaHCO3,the expression of PsGS was significantly influenced,which suggested that PsGS might play an important role in alkali salt stress resistance.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it