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Record W2388119900

Study on Agrobacterium-Mediated Transformation of Maize Callus

2011· article· en· W2388119900 on OpenAlexvenueno aff
Shihuang Zhang

Bibliographic record

VenueSeed · 2011
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicPlant tissue culture and regeneration
Canadian institutionsnot available
Fundersnot available
KeywordsCallusTransformation (genetics)AgrobacteriumTransformation efficiencyHorticultureInbred strainAgrobacterium tumefaciensBiologyBotanyGeneMolecular biologyGenetics
DOInot available

Abstract

fetched live from OpenAlex

An optimized transformation system was developed for maize elite inbred lines using Agrobacterium tumefaciens mediated gene transfer by studying various important factors which affect transformation efficiency.The system was performed by transient expression of GUS gene after embryogenic callus were placed on cocultivation medium for 3 days.The study with the embryogenic callus derived from immature embryos of maize inbred line Qi 319 and R 18-599 showed that the quality of the callus played an important role in the transformation process,the callus subcultured two times and pre-cultured for 7 days were the best for transformation.In addition,the study showed that the combination of EHA 105 and Qi 319 was better than EHA 105 and R 18-599 combination for transformation,which was concluded from the detection of transient GUS expression,since the average frequency of transient GUS expression of EHA 105-Qi 319(mean=31.20%) was significantly higher than that of EHA 105-R 18-599(mean= 23.74%) based on the test(t0.05).

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.001
Threshold uncertainty score0.003

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.022
GPT teacher head0.230
Teacher spread0.208 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2011
Admission routes1
Has abstractyes

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