Construction and Characterization of Enriched Microsatellite Library from Rehmannia glutinosa
Bibliographic record
Abstract
The DNA fractions containing microsatellite sequences were captured by hybridizating the digested Rehmannia glutinosa genomic DNA fragments with the oligonucleotide probes(AC)8,(AG)8 and(ATG)12 attached to striptavadin coated magnetic beads(Dynal).The enriched DNA fragments were ligated into pMD 18-T easy vector and then transformed into competent Trans 5 αcell to form an enriched microsatellite sequence library.PCR screening using M 13 Forward and M 13 Reverse as primers identified 96 clones containing 400-800 bp inserts.Sequence analysis of these positive clones confirmed 48 microsatellite sequences,with an enrichment efficiency of 50%.Of these microsatellite loci,66% were perfect repeat motif,22% imperfect and 12 compound repeat motifs.(AC)n and(CAT)n was the most common motif.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.002 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".