Impact of Surfactants on Asymmetric Bioreduction of 2-Octanone by Saccharomyces cerevisiae
Bibliographic record
Abstract
In aqueous buffer system containing surfactants of SDS,CTAB and Emulsifier OP-6,the asymmetric reduction of 2-octanone to(S)-2-octanol with Saccharomyces cerevisiae cells was studied.The results indicated that the yield and product e.e.were disappointingly low due to the serious reverse reaction and side reaction in the surfactant-free system.Particularly,at the substrate concentration of 10 mmol/L,they were 77.4% and 78.8% for 96 h reaction.With addition of surfactants to the system,the rates of reverse and side reactions were decreased by Emulsifier OP-6 and CTAB,with the effective increase of the average positive reaction rate.In the systems containing 0.4 mmol/L Emulsifier OP-6 or 0.04 mmol/L CTAB,the yield and e.e.value were 88.4%,91.1% and 97.0%,94.5%,respectively,obviously higher than those in the reaction system without surfactant.The further work showed that the enhancement of efficiency and enantioselectivity of the asymmetric reduction resulted from the effect of the surfactants on the cell characteristics such as growth,membrane penetrability,etc.Among the surfactants investigated,Emulsifier OP-6 performed best.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.003 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".