Expression of squalene synthase gene in Eleutherococcus senticosus and its influence on saponins content
Bibliographic record
Abstract
To know characteristics of expression of squalene synthase(SS) gene in Eleutherococcus senticosus and its influence on saponins content,taking GAPDH gene as reference gene,analyze expression of SS gene in different organs of E.senticosus during different growth periods was analyzed by using real time quantitative PCR technique.And influence of methyl jasmonate(MeJA) on expression of SS gene was researched.Relationship of expression of SS gene and total content of saponins in E.senticosus was analyzed by using SPSS 17.0 software.The results indicate that in the whole growth period,expression of SS gene shows the trend of low-high-low-high-low.Expression of SS gene reaches the lowest value at germination stage(April 26),and reaches the highest value at full flowering stage(June 26),which is 3.11 times as much as the former.Expression of SS gene in root is significantly higher than that in stem,leaf and petiole(P 0.05),and that value in stem is the lowest,which is 51.88% of the value in root.MeJA can enhance expression of SS gene,but the difference is insignificant.Both expression of SS gene and total saponins content have the same change trend,and they have very significantly positive correlation(P 0.01).According to the results,SS is a key enzyme gene in the biosynthesis of saponins in E.senticosus.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".