Preparation of the monoclonal antibody against VP6 in group A porcine rotavirus and its antigenic epitope identification
Bibliographic record
Abstract
To prepare the monoclonal antibody(MAb) against VP6 protein of group A porcine rotavirus(RV) and identify the antigenic epitope, a hybridoma stable secreting MAb against VP6 protein was prepared by fusing SP2/0 cells with spleen cells from BALB/c mice immunized with recombinant VP6 protein. Identification of the MAb indicated that it belonged to Ig G1 subtype withκ chain. The titers in cell culture medium of the hybridoma and ascites were 1 ∶12 800 and 1 ∶106, respectively. In addition, the MAb was positively reacted with authentic VP6 protein of porcine RV detected by western blot and indirect immunofluoresence assay. Moreover, the antigenic epitope sequence of134DYIENWNLQNR144 was identified by the pepscan technique. Those results provided a basis for the further study on the function of VP6 and development of diagnostic method.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".