Analysis of Several Factors that Affect Isolated Microspore Embryogenesis in Brassica napus
Bibliographic record
Abstract
Isolated microspore culture has greatly contributed to rapeseed haploid breeding,and is thus one of the most important technologies in rapeseed breeding and genetic engineering.Many parameters show effects on the in vitro microspore morphogenesis,and difficulties are encountered especially when microspore is collected directly from field-grown plants.The present isolated microspore culture used field-grown F1 plants from 40 cross combinations,in which 9 cross combinations produced embryos in culture(22.5% of total tested cross combinations),and plants regenerated in 4 cross combinations among them(10% of total tested cross combinations).The ratio of the embryogenesis of the 4 cross combinations ranged 0.08-0.86 embryo/bud,at an average of 0.25 embryo/bud.Cytological analysis showed that the stage of flower bud at 3.5mm in length gave rise to the best embryogenesis.Branches with rachises collected from field-grown plants could be stored at 4 ℃ for 4-5 days in room light that facilitated lab analysis and culture.The percentage of microspore enlargement at 2 days after heat shock culture is an important marker for embryogenesis.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".