The Study of High Oleic Acid Rapeseed Disease Resistance Related Genes by Transcriptome and iTRAQ Analysis
Bibliographic record
Abstract
There are obvious differences between high oleic acid rapeseed and low oleic acid rapeseed on sclerotinia sclerotiorum resistance. In order to find out the molecule mechanism,the high oleic acid rapeseed inbred line seeds 20-35 d after pollination were used as material for transcriptome analysis and i TRAQ analysis respectively in this study. The classifications associated with disease resistance were oxidative phosphorylation,plant hormone signal transduction and plant-pathogen interaction were discussed,and the relationship between differential genes and the corresponding protein were investigated too. Then real-time quantitative PCR( q PCR) analysis was used to verify the expression levels of differentially expressed genes which may be associated with disease resistance. Combined with previous study,the genes related with disease resistance were: gi | 260505503( polygalacturonase inhibitory protein),gi | 226346102( HSR203J-like protein) and gi | 470103214( caltractin-like). And the genes of gi | 297843222( binding protein),gi | 18397961( 2Fe-2S ferredoxin-like protein),gi | 196052306( NADH dehydrogenase subunit),gi |18423437( NADH dehydrogenase( ubiquinone) 1alpha subcomplex 5) and gi | 297794581( kinase family protein)have obvious difference.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".