Study on Double PCR System Based on Molecular Marker of Ogura-CMS Restorer Lines in Brassica Napus L.
Bibliographic record
Abstract
Ogura-CMS of Brassiais Napus L.,one of the most stable infertility materials,caused by Orf 138 chimeric genes of mitochondria and a pair of recessive gene(rfrf)of nucleus,is an effective way for heterosis utilization in Brassica napus L.According to the nucleotide sequences of Orf 138 infertility genes and its restorer genes Orf 687,two pair of special PCR primers were designed respectively,the double PCR system was established to amplify target bands for the two genes using Ougra-CMS lines,maintainer lines,restorer lines.The results showed that the double PCR system established was stable and reliable in the markers assisted selection of restorer lines for F2 generation,and the selection results were thoroughly coincident with conventional testcross results,and the selection efficiency was improved.This study provides a practical and reliable double-PCR marker technological system for the assisted selection of Brassica napus Ougra CMS restorer lines.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".