Transgenic Research of A3 Promoter-Deficient piggyBac-derived Transposon in the Silkworm,Bombyx mori
Bibliographic record
Abstract
We constructed the plasmid harbored the A3 promoter-deficient piggyBac-derived transposon and the enhanced green fluorescence protein(EGFP) gene.The constructed plasmid was microinjected into the eggs of Nistari strain,along with the helper plasmid.Transgenic experiment results showed that the transformation efficiency was high,and the rate of number of G0 moths whose offspring expressed the EGFP to the number of eggs injected was 0.618%.Moreover,EGFP was expressed with the manner of specific tissue,and the positive moths are easy to be observed in G0 generation.The transgenic silkworms were also verified by PCR method.Our results indicated that A3 promoter-deficient transformation in the silkworm maybe used to screen the promoter activated in specific tissue.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.002 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".