Dicationic gemini surfactant gene delivery complexes contain cubic-lamellar mixed polymorphic phase
Bibliographic record
Abstract
The characterization of structural properties of novel dicationic (gemini) surfactant-based DNA complexes as micro/nano-scale self-assembling delivery systems for cutaneous gene therapy is described and discussed as related to measured transfection efficiencies. We have identified the Pn3m cubic phase in DNA-gemini-DOPE complexes with gemini surfactants having 12, 16 and 18:1 alkyl tail length. Increasing gemini/DNA charge ratios (ρ+/-) from 0.5 to 10 resulted in increasingly mixed (Pn3m and HII or Pn3m and L) polymorphic systems with lamellar (L) features becoming more predominant. DNA-gemini complexes exhibited very weak single scattering peaks representative of gemini- plasmid particles with no long range order and low transfection efficiency. In gemini – DOPE complexes the main complex geometry is inverted hexagonal (HII) at low concentrations of gemini, with weakly ordered systems (generally lamellar) observed at increased concentrations. Overall, the presence of the newly identified cubic Pn3m phase in the DNA-geminiDOPE complexes appears to be advantageous for increased transfection efficiency.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".