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Record W2397955395 · doi:10.5376/mpb.cn.2012.10.0070

甘薯块根储藏蛋白(Sporamin)启动子克隆及功能验证

2012· article· zh· W2397955395 on OpenAlexvenueno aff
张聪

Bibliographic record

Venue分子植物育种 · 2012
Typearticle
Languagezh
FieldEngineering
TopicBiofuel production and bioconversion
Canadian institutionsnot available
Fundersnot available
KeywordsChemistry

Abstract

fetched live from OpenAlex

提取甘薯“川薯34”总DNA,设计引物,通过高保真PCR扩增获得了长为1 144 bp的甘薯储藏蛋白(Sporamin)基因启动子SpoA-p。PLACE在线分析表明:Sporamin启动子具有基本的启动子元件CAAT- box,并包含大量与储藏蛋白表达相关、抗逆相关及蔗糖表达相关的功能组件。构建植物表达载体pBISpoA-p::GUS、农杆菌工程菌株EHA105:pBI-SpoA-p::GUS后导入烟草及甘薯,分别得到15株烟草和10株甘薯的阳性转基因植株,GUS染色分析表明烟草各部位中只在根中有基因表达,甘薯根、茎和叶各部位未检测到活性。5%蔗糖诱导处理24 h后,在烟草根、茎及叶均观察到GUS活性;在甘薯中只有根有GUS活性。因此,可以看出此启动子为甘薯根部特异的表达启动子,并受蔗糖诱导而表达。

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.003
Threshold uncertainty score0.011

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.001
Science and technology studies0.0010.001
Scholarly communication0.0010.001
Open science0.0000.000
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0030.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.016
GPT teacher head0.213
Teacher spread0.197 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2012
Admission routes1
Has abstractyes

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