Production of Haploid and Diploid Androgenetic Zebrafish
Bibliographic record
Abstract
Zebrafish (Danio rerio) are a popular vertebrate model system, particularly useful for research in developmental genetics and neurobiology. The adults are easy to obtain and rear; the generation times are only a few months; and large clutches of big embryos are produced (100–1000 per mating). The embryos develop externally and are nearly transparent, facilitating microscopy, experimental manipulations, and screening for morphological mutants. High-density genetic linkage maps have been produced for visible and DNA markers, and the genome is being sequenced. Several useful genetic tools have been developed. Large-scale mutagenesis screens have detected genes involved in morphogenesis and other developmental processes (1,2). These screens involved a classical three-generation crossing strategy to detect recessive lethal mutations revealed in homozygous diploid mutants. Similar screens are done using insertional mutagenesis to facilitate cloning of the disrupted genes (3). Many useful molecular markers of differentiating cells have been characterized, and gene expression can be effectively manipulated by the use of morpholino antisense oligonucleotides or expression of dominant-negative mutant proteins. Much information concerning zebrafish mutants, strains, and methods can be found at http://www.zfin.org .
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".