PCR Screening in Signature-Tagged Mutagenesis of Essential Genes
Bibliographic record
Abstract
Signature tagged-mutagenesis (STM) is a functional genomics technique that identifies microbial genes required for infection within an animal host, or within host cell (,). As first described by Hensel et al., 1995 (), transposon mutants are generated and each one tagged with a unique DNA sequence. Originally, STM used comparative hybridization to isolate mutants unable to survive in specified environmental conditions and to identify genes critical for survival in the host (). The original STM has been modified to use defined oligonucleotides for tag construction into mini-Tn5 and to use polymerase chain reaction (PCR) instead of hybridization for rapid screening of bacterial mutants in vivo (). The modified STM technique has been called PCR-based signature-tagged mutagenesis (PBSTM).
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".