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Record W2407645185 · doi:10.1385/1-59259-227-9:229

Detection of EBV Latent Proteins by Western Blotting

2003· article· en· W2407645185 on OpenAlexaff
Martin Rowe, Matthew Jones

Bibliographic record

VenueHumana Press eBooks · 2003
Typearticle
Languageen
FieldMedicine
TopicViral-associated cancers and disorders
Canadian institutionsInstitute of Infection and Immunity
Fundersnot available
KeywordsBlotNitrocellulosePolyacrylamide gel electrophoresisGel electrophoresisMolecular biologyChemistryPolyacrylamideChromatographyReagentElectrophoresisMembraneBiologyBiochemistry

Abstract

fetched live from OpenAlex

Western blotting is a well established technique for identifying Epstein-Barr virus (EBV)-encoded proteins in lysates from cell lines or biopsy material. The basic technique involves separation of proteins by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and transfer onto a support membrane such as nitrocellulose or polyvinylidine difluoride (PVDF), followed by immunostaining with specific antibody reagents. The initial SDS-PAGE separation procedure is essentially similar to the method originally described by Laemmli (1), except that vertical slab gels are used instead of the original tube gels. Until recently, the slab gels commonly used were relatively large (approx 16×16 cm), but now it has become popular to use “mini” slab gels (approx 7×8 cm) because the electrophoresis and blotting times are considerably reduced and there is a significant saving in the amounts of the reagents required.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.338
Threshold uncertainty score0.384

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.034
GPT teacher head0.266
Teacher spread0.232 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations11
Published2003
Admission routes1
Has abstractyes

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