Influence of threonine intake on whole-body protein deposition and threonine utilization in growing pigs fed purified diets.
Bibliographic record
Abstract
The relationship between available threonine (Thr) intake and whole-body protein deposition (PD) was established using the serial slaughter method in 36 individually housed growing gilts between 39 and 77 kg live BW. Pigs were prescreened for their maximum PD (PDmax), based on a N balance starting at 25 kg BW while they consumed semi-ad libitum a nonlimiting diet. Pigs were fed combinations of a casein and cornstarch-based diet that was confirmed to be first-limiting in Thr and a protein-free diet starting at approximately 30 kg BW. Casein-bound Thr was provided at 60, 70, 80, 90, 100, or 120% of estimated Thr requirements for PDmax. Energy intake was kept constant across treatments and exceeded requirements for PDmax. Pigs were fed three equal meals per day; feeding levels were adjusted weekly based on BW. Pigs were killed at either 39 kg BW (n = 2 per treatment) or 77 kg BW (n = 4 per treatment) for determining chemical body composition. Composition of 39-kg BW pigs was not different across treatments (P > 0.10); therefore, an overall mean initial body composition was used to estimate body protein content at the initial BW. Across treatments, mean daily ME intake was 25.3 (SE 0.08) MJ/d and did not differ (P > 0.10) among treatments. Average daily true ileal digestible Thr intake varied between 5.33 and 9.66 g/d, representing means for pigs on the lowest and the highest Thr intakes, respectively. Mean PD was 93, 102, 118, 124, 139, and 133 (SE 4.2) g/d for pigs on the six respective treatments. Dietary Thr intake did not influence (P > 0.10) Thr content of body protein at the final BW or the partitioning of body protein between carcass, viscera, and blood. The efficiency of Thr utilization for PD was lowest (P < 0.05) at the highest Thr intake level and highest (P < 0.05) at the lowest Thr intake level. It was similar (P > 0.10) at the four intermediate Thr intake levels, in which the relationship between true ileal digestible Thr intake and PD was linear. Based on these four treatments, calculated Thr disappearance, which is closely associated with inevitable Thr catabolism, was 23.5 (SE 0.55)% of available Thr intake. This value is consistent with an efficiency of using available Thr intake above maintenance Thr requirements (54 mg/kg BW0.75) for Thr retention with PD of 73.4 (SE 1.11)%. Based on N balances conducted at approximately 40 and 75 kg BW, the marginal efficiency of Thr utilization was not influenced by BW.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.001 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".