On the Statistics of Identifying Candidate Pathogen Effectors
Bibliographic record
Abstract
High-throughput sequencing is an increasingly accessible tool for cataloging gene complements of plant pathogens and their hosts. It has had great impact in plant pathology, enabling rapid acquisition of data for a wide range of pathogens and hosts, leading to the selection of novel candidate effector proteins, and/or associated host targets (Bart et al., Proc Nat Acad Sci U S A doi:10.1073/pnas.1208003109, 2012; Agbor and McCormick, Cell Microbiol 13:1858-1869, 2011; Fabro et al., PLoS Pathog 7:e1002348, 2011; Kim et al., Mol Plant Pathol 2:715-730, 2011; Kimbrel et al., Mol Plant Pathol 12:580-594, 2011; O'Brien et al., Curr Opin Microbiol 14:24-30, 2011; Vleeshouwers et al., Annu Rev Phytopathol 49:507-531, 2011; Sarris et al., Mol Plant Pathol 11:795-804, 2010; Boch and Bonas, Annu Rev Phytopathol 48:419-436, 2010; Mcdermott et al., Infect Immun 79:23-32, 2011).Identification of candidate effectors from genome data is not different from classification in any other high-content or high-throughput experiment. The primary aim is to discover a set of qualitative or quantitative sequence characteristics that discriminate, with a defined level of certainty, between proteins that have previously been identified as being either "effector" (positive) or "not effector" (negative). Combination of these characteristics in a mathematical model, or classifier, enables prediction of whether a protein is or is not an effector, with a defined level of certainty. High-throughput screening of the gene complement is then performed to identify candidate effectors; this may seem straightforward, but it is unfortunately very easy to identify seemingly persuasive candidate effectors that are, in fact, entirely spurious.The main sources of danger in this area of statistical modeling are not entirely independent of each other, and include: inappropriate choice of classifier model; poor selection of reference sequences (known positive and negative examples); poor definition of classes (what is, and what is not, an effector); inadequate training sample size; poor model validation; and lack of adequate model performance metrics (Xia et al., Metabolomics doi:10.1007/s11306-012-0482-9, 2012). Many studies fail to take these issues into account, and thereby fail to discover anything of true significance or, worse, report spurious findings that are impossible to validate. Here we summarize the impact of these issues and present strategies to assist in improving design and evaluation of effector classifiers, enabling robust scientific conclusions to be drawn from the available data.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.002 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".